Low-Voltage FESEM Imaging of Biofilms

Carlos Munoz, Ph.D. a candidate in the chemical and biochemical engineering department (Western University), used our field-emission scanning electron microscope (FE-SEM) to image his biofilm samples at low accelerating voltages (e.g., 1.5 keV). These low voltages allow very gentle imaging of the cells, and provides very surface sensitive analyses. The attached images show a biofilm of Shewanella oneidensis on a carbon felt electrode used in Microbial Fuel Cells. In this biofilm, the detailed nanowires are connections between cells.